水稻原胚和刚启动分化的幼胚cDNA文库的构建与分析

摘要

Two cDNA libraries were constructed from microdissected 214 rice proembryos (2-3 d after pollination) and 121 just differentiating young embryos (3-5 d after pollination) respectively through RT_PCR technique. The primary libraries had a total of 3.7×10 6 phages for the proembryos and a total of 2.5×10 6 phages for the just differentiating young embryos, in which 96% of the phages were recombinants. Insert sizes ranging from 400 bp to 3?500 bp were obtained. All of the above mentioned accorded with the general requirements of cDNA library construction.