A mRNA whole_mount in situ hybridization method is reported here for quick, direct analysis of the spatial and temporal mRNA expression patterns in plant young embryos. A cDNA clone THE 3 (tobacco heart embryo 3) was isolated by differential screening from tobacco (Nicotiana tabacum L.) heart embryo cDNA library as compared with the globular embryo cDNA library. The distribution of THE 3 mRNA in tobacco heart embryos and globular embryos was investigated by a whole_mount in situ hybridization technique, showing that THE 3 is preferentially expressed in heart embryos.
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