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Overexpression of Sweet Pepper Glycerol-3-Phosphate Acyltransferase Gene Enhanced Thermotolerance of Photosynthetic Apparatus in Transgenic Tobacco

机译:甜椒甘油3-磷酸酰基转移酶基因的过量表达增强了转基因烟草光合装置的耐热性。

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摘要

In order to investigate the relationship between the lipid composition in thylakoid membrane and thermostability of pho-tosynthetic apparatus, tobacco transformed with sweet pepper sense glycerol-3-phosphate acyltransferase (GPA T) gene were used to analyze the lipid composition in thylakoid membrane, the net photosynthetic rate and chlorophyll fluorescence parameters under high temperature stress. The results showed that the saturated extent of monogalactosyldiacylglycerol (MGDG), suifoquinovosyldiacylglycerol, digalactosyldiacylglycerol and phosphatidylglycerol in thylakoid membrane of transgenic tobacco T1 lines increased generally. Particularly, the saturated extent in MGDG increased obviously by 16.2% and 12.0% in T1-2 and T1-1, respectively. With stress temperature elevating, the maximum efficiency of photosystem Ⅱ the two lines and wild type tobacco plants decreased gradually, but those parameters decreased much less in transgenic plants. Even though the recovery process appeared differently in the donor and acceptor side of PSII in transgenic tobacco compared with wild-type plants, the entire capability of PSII recovered faster in transgenic tobacco, which was shown in Increase in saturated extent of thylakoid membrane Iipids in transgenic plants enhanced the stability of photosynthetic apparatus under high temperature stress.
机译:为了研究类囊体膜中脂质组成与光合装置热稳定性之间的关系,采用甜椒感性3-磷酸甘油酰基转移酶(GPA T)基因转化的烟草,分析类囊体膜中的脂质组成,高温胁迫下的净光合速率和叶绿素荧光参数结果表明,转基因烟草T1品系类囊体膜中单半乳糖基二酰基甘油(MGDG),硫代喹啉基二酰基甘油,二半乳糖基二酰基甘油和磷脂酰甘油的饱和度普遍增加。特别是,MGDG的饱和度在T1-2和T1-1中分别明显增加了16.2%和12.0%。随着胁迫温度的升高,两系和野生型烟草植物的光系统Ⅱ的最大效率逐渐降低,而转基因植物中这些参数的降低幅度要小得多。尽管与野生型植物相比,转基因烟草中PSII的供体和受体侧的恢复过程有所不同,但PSII的全部功能在转基因烟草中的恢复速度更快,这表明转基因烟草中类囊体膜脂质的饱和程度增加植物增强了高温胁迫下光合装置的稳定性。

著录项

  • 来源
    《植物学报(英文版)》 |2008年第5期|613-621|共9页
  • 作者单位

    College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China;

    College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China;

    College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China;

    College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China;

    College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China;

    College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 植物学;
  • 关键词

    glycerol-3-phosphate transferase; lipids in thylakoid membrane; photosynthesis; tharmotolerance; tobacco.;

    机译:3-磷酸​​甘油转移酶;类囊体膜中的脂质;光合作用;鸡耐受性烟草。;
  • 入库时间 2024-01-27 07:48:31
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