Amino Acids Analysis in Different Antigens and Relationship with Immunoreactivity in Cysticercus cellulosae

摘要

The six antigens of Cysticercus cellulosae: CFag, CSag, CBWag, CWag, UCWag and TSag were prepared respectively. The amino acids of the antigens were determined quantitative by automatic amino acid analyzer. The relationship of original reaction was confirmed between amino acids and antigens. The results showed that there were 17 amino acids among all of the antigens. There were no significant difference (P >0.05) of Asp, Glu, Lys, His in composition of all antigens by data analysis software SPSS. There are also no significant difference ( P ＞ 0.05) in the composition of all amino acids between CSag and CBWag. The composition of Thr, Ser, Tyr, Ved, Pro in UCWag shows significant difference (P ＜ 0.05) with other antigens.The sensitivity and peculiarity of UCWag are higher than that of the others. Pro and Glu show significant linear correspondence with sensitivity and peculiarity of antigens respectively.