一种基于角锥棱镜的上转换荧光检测系统

摘要

Upconversional fluorescence materials receive more and more attentions, but their applications suffer from the low conversional efficiency of fluorescence. In other words, it makes precise quantification difficult. In this study, a cube-corner retroreflector was introduced to collect more fluorescence from upconversional materials. A prototyping upconversional fluorescence detection system was developed with a dual optic path containing a cube-corner retroreflector and a coaxial multicore fiber for accepting fluorescence from both sides of the cube-corner retroreflector. A series of experiments were carried out to calibrate this prototyping system. It is found that the dual optic path can collect more fluorescence, and thus it improves the detection efficiency of weak fluorescence. The results confirm the feasibility of the upconversional fluorescence detection system with cube-corner retroreflector.%上转换荧光材料在很多领域都具有新颖的应用潜力，然而其较低的发光效率给定量检测带来了难度。文章针对上转换荧光材料发光效率较低的特点，利用角锥棱镜的光学特性，设计了一套新型的上转换荧光检测光路。该装置将传统单工荧光检测光路扩展为双工系统，利用同轴多芯光纤从角锥棱镜两端同时接收荧光，提高了检测系统对微弱荧光的采集效率。在本研究中，设计了一系列实验来检验双工荧光收集光路的可行性，量化了荧光检测系统的线性度，从而证明了基于角锥棱镜上转换荧光检测系统的可行性。