动态吸附装置与高效液相色谱-串联质谱联用测定花生植株中的茉莉酸甲酯与水杨酸甲酯

摘要

A novel method was developed for the qualitative and quantitative analysis of trace volatile organic compounds(VOCs), methyl jasmonate and methyl salicylate, emitted from peanut based on a designed dynamic adsorption device coupled with high performance liquid chromatography tandem mass spectrometry( HPLC -MS/MS). The VOCs were adsorbed with Tenax-TA, eluted with methanol solution containing 0.1％ formic acid, and concentrated with nitrogen. The separation of the VOCs was carried out on an Agilent C18 column by gradient elution mode using methanol -0. 05％ formic acid as mobile phase at a flow rate of 300 μL/min. The samples were analyzed by ion trap mass spectrometer under positive ion mode with multiple reaction monitoring. The internal standard method was used for quantitative analysis. The detection limits ( S/N= 3 ) for methyl jasmonate and methyl salicylate were 2.0 μg/L and 20. 0 μg/L, and the quantitation limits( S/N = 10)were 5.0 μg/L and 50.0 μg/L, respectively. There was a good linear relationship(r ＞0. 98) between the concentration and the peak area ratio in the range of 1.0 -500 μg/L for methyl jasmonate and 20 - 1 000 μg/L for methyl salicylate. The method was simple, rapid and high efficient, and was suitable for the investigation of changes of volatile organic compounds emitted from plant at different treatment conditions.%将设计组装的动态吸附装置与高效液相色谱-串联质谱联用建立了花生植株中痕量挥发物茉莉酸甲酯与水杨酸甲酯的测定方法.采用Tenax-TA吸附剂吸附挥发物,0.1%甲酸-甲醇溶液洗脱,洗脱液浓缩后进样.色谱柱为Agilent C18柱(2.1mm×150mm,5μm),以甲醇和0.05%甲酸为流动相,流速300μL/min,梯度洗脱.采用多反应监测(MRM)正离子模式测定,内标法定量.茉莉酸甲酯与水杨酸甲酯的检出限(S/N=3)分别为2.0、20.0μg/L,定量下限(S/N=10)分别为5.0、50.0μg/L;在一定范围内峰面积比与质量浓度的线性关系良好(r＞0.98).该方法简便快捷、富集效率高,可用于植株在不同处理条件下痕量挥发物变化情况的研究.