纳米金胶增强葡萄糖生物传感器的构建

摘要

将纳米金胶(AuNPs)和羟基磷灰石(HAp)按一定比例混合制备了新型复合膜用于葡萄糖氧化酶(GOD)的固定,构建了高灵敏的葡萄糖传感器.由于纳米金胶的存在,葡萄糖氧化酶的直接电化学性质得以增强,在去除氧气的PBS( pH 7.0)介质中,固定在复合膜内的GOD表现出一对良好的氧化还原峰.在饱和氧气条件下,当加入一定量的葡萄糖时,由于GOD催化葡萄糖氧化消耗溶液中的溶解氧,-0.8V处溶解氧的还原峰电流降低,且峰电流降低的量与葡萄糖浓度在0.02～1.62 mmol/L范围内呈线性相关,检出限为5.0 μmol/L,检测灵敏度达9.91 mA·mol-1·L,可实现对葡萄糖的快速检测.%A highly sensitive glucose biosensor was fabricated by immobilizing the glucose oxidase (GOD) on a new hybrid film of Au nanoparticles( Au NPs) and hydroxyapatite( HAp). The direct electrochemical behavior of GOD in the hybrid film was enhanced and a pair of well-defined, nearly reversible peaks appeared in deoxygenized pH 7. 0 PBS due to the presence of AuNPs. The experi-mental results also showed that the peak current of dissolved oxygen at - 0. 8 V decreased with the addition of glucose due to the consumption of dissolved oxygen when the glucose was oxidized by GOD. The decreased reduction current of oxygen was linear with concentration of glucose in the range of 0.02-1.62 mmol/L with a sensitivity of 9. 91 mA mol-1 L and a detection limit of 5. 0 μmol/ L. The biosensor could be applied in the determination of glucose with high sensitivity and speedi-ness.