A new type of solid phase extraction column(Bond Elut ENV) was used for the rapid determination of benzo [ a ] pyrene in edible vegetable oil. And a solid phase extraction purification and high performance liquid chromatography/fluorescence detection method was developed for the determination of benzo[a] pyrene in edible vegetable oil. Samples were dissolved in n-hexane and then purified by solid phase extraction. Chromatographic analysis was performed on a SUPELCOSILTM LC -PAH(25 cm×4. 6 mm, 5 μm) column using acetonitrile water (95:5) as mobile phase. Quantitation analysis was carried out by the external standard method using fluorescence detecter at λex = 297 nm and λem =408 nm. The calibration curve was linear over the benzo[ a] pyrene concentration in the range of 1.0 - 50. 0 μg/L (r = 0. 999 6) with detection limit of 0. 3 μg/kg. The recoveries ranged from 79% to 102% with relative standard deviations no more than 9. 4% . The method is accurate, practical, simple and fast, and has an extensive application prospect in the determination of benzo [a] pyrene residues in edible vegetable oil.%研究了Bond Elut ENV新型固相萃取柱在食用植物油中苯并[a]芘快速检测中的应用,建立了快速测定食用植物油样品中苯并[a]芘残留量的固相萃取/液相色谱/荧光检测法.样品用正己烷溶解,固相萃取净化,SUPELCOSILTM LC - PAH(25 cm×4.6 mm,5μm)色谱柱分离,以乙腈-水(95:5)为流动相,荧光检测(λ(n) =297 nm,λem=408 nm),外标法定量.苯并[a]芘的检出限为0.3μg/kg,在1.0 ～50.0 μg/L范围内线性关系良好,相关系数为0.9996,方法的回收率为79％～102％,相对标准偏差不高于9.4％.该方法准确、实用、简便、快速,在食用植物油的苯并[a]芘残留量检测方面有广泛的应用前景.
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