A rapid method for the determination of glyceryltrioleate was developed by using screenprinted electrode ( SPE) modified with lipase as an electrochemical biosensor. Cyclic voltammetry (CV) and chronocoulometry(CC) were applied to characterize the behavior of glyceryltrioleate on the surface of the lipase-modified SPE. The optimal conditions of glyceryltrioleate determination were investigated. A linear response to glyceryltrioleate in the concentration ranges of 0. 01 -5 g- L-1 with a detection limit of 2. 5 mg L-1 for CV, and 0. 001 - 5. 50 g L-1 with a detection limit of 0. 2 mg ·L-1 for CC were obtained. The method was used in the determination of 6 sunflower seed oil samples with spiked recoveries of 94% -105% .%制备了用于快速检测三油酸甘油酯的脂肪酶修饰丝网印刷电极(SPE)电化学传感器单元.用循环伏安法(CV)和计时电流法(CC)研究了三油酸甘油酯在脂肪酶修饰SPE上的电化学行为,确定了测定甘油酯的最优条件,建立了测定三油酸甘油酯浓度的电化学方法.其中CV法的线性范围为0.01~5 g,L-1,检出限为2.5 mg· L-1; CC法的线性范围为0.001~5.50 g·L-1,检出限为0.2 mg· L-1.以CV法考察样品的加标回收率,6个葵花籽油样品的加标回收率为94% ~ 105%.
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