市售不同产地辛夷中挥发油成分分析及木兰脂素的含量测定

摘要

Objective To study the chemical component of volatile oil of Flos Magnoliae from Hunan and Guangxi, and the magnolin content was determined. Methods The volatile oil of the Flos Magnoliae in Hunan and Guangxi was analyzed by GC-MS, and the content of magnolin was determined by HPLC. Results 63 and 65 compounds were respectively separated and identified from Flos Magnoliae volatile oil that was collected from Hunan and Guangxi, mainly including eucalyptol, beta pinene, 2-camphor, geranyl linalool, alpha terpineol etc. Magnolin presented a good linearity with peak area between the concentration of 2-256 μg/mL (r=0.99996, n=6). The average recovery was 100.32% with the RSD of 0.04%. The content of Magnolia from the province of Hunan and Guangxi was 0.87%±0.05% and 0.81%±0.02%. Conclusion GC-MS can detect the composition of essential oil quickly and effectively. The analysis of magnolin with HPLC was simple, reliable and reproducible.%目的：分析比较市售湖南、广西两个不同产地辛夷中挥发油的化学成分，并对其木兰脂素的含量进行测定。方法采用GC-MS联用技术对湖南、广西两个产地的辛夷挥发油成分进行分析，采用HPLC对两个产地辛夷中木兰脂素含量进行测定。结果湖南、广西产地的辛夷挥发油分别分离鉴定出62和64种化合物，主要成分为桉油精、β-蒎烯、2-茨酮、香叶基芳樟醇、α-松油醇等。采用HPLC测定辛夷中木兰脂素的含量，结果显示，木兰脂素在2~256μg/mL浓度范围内与峰面积呈良好线性关系（r=0.99996，n=6），平均加样回收率为100.32％，RSD为0.04％（n=6）。木兰脂素含量为：湖南产地（0.87±0.05）%、广西产地（0.81±0.02）%。结论 GC-MS法能快速、简便、高效地检测辛夷挥发油的化学成分，HPLC简便可靠、重复性好，可用于辛夷中木兰脂素的含量测定。