柴胡三参胶囊对缺血性心律失常大鼠模型心肌细胞HERG K+通道蛋白的研究

摘要

Objective To investigate Chaihu Sanshen capsule on myocardial ischemic arrhythmia mice HERG K +channel protein expression in ischemic arrhythmia rat models, and provide more experimental basis for clinical use of Chaihu Sanshen capsule. Methods The rats were randomly divided into blank group, sham-operation group, model group, Chaihu Sanshen (Qinghao) group, Chaihu Sanshen (Changshan) group, Wenxin granule group, amiodarone group, 10 rats in each group. The drug group was given pretreatment for 10 days before 10 days of left anterior descending coronary artery. The change of electrocardiogram and myocardial HERG K + channel protein expression in left anterior descending coronary artery was observed. Results Chaihu Sanshen capsule can reduce the incidence of ischemic arrhythmias (P<0.05), and significantly reduce the HERG K+ channel protein inactivation (P<0.01). Conclusion The antiarrhythmic effect of Chaihu Sanshen capsules is obvious, and its targets are HERG K+ channel proteins.%目的：探讨柴胡三参胶囊对缺血性心律失常大鼠心肌中HERG K+通道蛋白表达的影响，为柴胡三参胶囊的临床应用提供实验依据，为缺血性心律失常的中医药治疗提供更多的治疗途径。方法将大鼠随机分为空白组、假手术组、模型组、柴胡三参胶囊（青蒿）组、柴胡三参胶囊（常山）组、稳心颗粒组、胺碘酮组，每组各10只，药物组在结扎左冠状动脉前降支前10 d开始预先给药，连续10 d，观察大鼠结扎左冠状动脉前降支后心电图改变及心肌中 HERG K+通道蛋白表达。结果柴胡三参胶囊能够降低缺血性心律失常的发生率(P<0.05)、显著性的降低大鼠心肌细胞HERG K+通道蛋白的失活(P<0.01)。结论柴胡三参胶囊抗缺血性心律失常的效果明显，心肌细胞HERG K+通道蛋白是其作用靶点。