舒肝解郁胶囊对抑郁模型大鼠脑内5-HT、DA及其代谢产物水平的影响

摘要

目的：研究舒肝解郁胶囊对抑郁模型大鼠脑内5-HT、DA 及其代谢产物水平的影响。方法将28只雄性 SD大鼠随机分成正常对照组、模型组、舒肝解郁组、氟西汀组四组；采用慢性轻度不可预见性应激(CUMS)结合孤养方式建立抑郁大鼠模型，舒肝解郁组和氟西汀组大鼠于造模同时分别给予舒肝解郁胶囊和氟西汀干预；用库仑阵列电化学高效液相色谱法分别检测大鼠内侧前额叶皮质(mPFC)及海马CA3区5-HT、DA 及其代谢产物的含量。结果应激21 d 后，模型组与正常对照组比较，大鼠 mPFC 区5-HT、5－羟吲哚乙酸(5-HIAA)、DA 和高香草酸(HVA)的浓度均显著降低，差异有统计学意义(P<0.01)，海马 CA3区5-HT、DA 和5-HIAA 的浓度均显著降低，差异有统计学意义(P<0.05)；与模型组比较，舒肝解郁组和氟西汀组可显著增加大鼠 mPFC 区5-HT 、DA 和 HVA 浓度，差异有统计学意义(P<0.01或 P<0.05)，海马 CA3区5-HT、 DA 和5-HIAA 浓度显著升高，差异有统计学意义(P<0.05)。结论舒肝解郁胶囊能增强抑郁模型大鼠中枢5－HT 和 DA 神经递质系统的功能，这可能是其抗抑郁作用的神经生化机制之一。%Objective To evaluate the effect of Shugan Jieyu (SGJY) capsules on 5-HT, DA and their metabolites in the brain of depression model rats. Methods Twenty-eight male SD rats were randomly divided into normal control group, model group, SGJY group and fluoxetine group. We established the depression rat models with the method of chronic unpredictable mild stress (CUMS) and separate feeding. The SGJY group anf fluoxetine group were intervened with SGJY capsule and fluoxetine. The 5-HT, DA and their metabolites levels in medial prefrontal cortex (mPFC) and hippocampal CA3 areas were detected by HPLC-ECD method. Results After 21 days' stress, compared with the control group, the levels of 5-HT, 5-hydroxyindoleacetic acid (5-HIAA), DA and homovanillic acid (HVA) in the mPFC of depression model rats were significantly decreased (P<0.01), and the levels of 5-HT, 5-HIAA and DA in hippocampal CA3 area were significantly decreased (P<0.05). Compared with model group, the levels of 5-HT, DA and HVA in the mPFC of fluoxetine and SGJY groups were significantly increased (P<0.01 or P<0.05), and the levels of 5-HT, DA and 5-HIAA in hippocampal CA3 area were significantly increased (P<0.05). Conclusion The SGJY capsule could increase the function level of DA and 5-HT neurotransmitter systems in mPFC and hippocampus areas of depression model rats.