蔓荆子总黄酮抑制肝细胞癌球形成与激活AMPK抑制AKT相关

摘要

Objective To investigate FructusViticis Total Flavonoids(FVTF) inhibits self-renewal capability of human hepatocellular carcinoma cells and the mechanism by which is involved in regulation of AMPK/Akt signaling. Methods Tu-morsphere formation assay was used to determine the effects of FVTF on sphere forming rate of hepatocellular carcinoma SMMC-7721 and MHCC97H cell lines. Western blot was emploied to analyze the phosphorylation levels of AMPK and Akt proteins of SMMC-7721 cells treated with FVTF. Results FVTF significantly lowered the sphere forming rates of SMMC-7721 and MHCC97H cell lines, in a concentration-dependent manner. Treatment with various concentrations of FVTF(1.0、2.0、4.0μg/mL) for 24 h, the phosphorylation level of AMPK was elevated and the phosphorylation level of Akt was reduced in SMMC-7721 cells. Conclusion FVTF inhibits self-renewal capability of hepatocellular carcinoma cells is associated with ac-tivation of AMPK and suppression of Akt.%目的：研究蔓荆子总黄酮（FVTF）抑制人肝细胞癌自我更新作用及其机制是否涉及AMPK/Akt信号传导。方法：肿瘤球形成法测定FVTF对肝细胞癌SMMC-7721和MHCC97H细胞系球形成率的影响。Western blot分析FVTF处理SMMC-7721细胞AMPK和Akt磷酸化水平。结果：FVTF显著降低SMMC-7721和MHCC97H细胞系球形成率，呈浓度依赖性。不同浓度FVTF（1.0、2.0、4.0μg/mL）处理SMMC-7721细胞24 h，AMPK磷酸化水平增高；Akt磷酸化水平下降。结论：FVTF抑制肝细胞癌自我更新作用与其激活AMPK抑制Akt活性相关。