B.Subtilis expression plasmids generally require a stringent Shine Dalgarno Sequence(SDS). Site directed mutagenesis was explored to change the Shine Dalgarno Sequence from AAAAATGGGG (mutant type) to AAAAAGGGGG (wild type) in recombinant plasmid PSM604. The single base substitution made the plasmid with wild SDS unstable in structure and segregation. The interaction of SDS with subtilisin leader sequence of PSM604 might be responsible for the instability of plasmid.
展开▼
机译:Translating Success and Happiness: A Study on Dale Carnegie’s How to Win Friends and Influence People in Chinese Translations =翻译成功,译介幸福:卡内基之How to Win Friends and Influence People译本研究