Immune Killing Activity of Lymphocytes on Hela Cells Expressing Interleukin-12 In Vitro

摘要

The killing effects of lymphocytes on Hela cells expressing interleukin-12(IL-12) in vitro were explored.By using gene transfection technique,full length IL-12 gene was transfected into Hela cells.The expression of IL-12 in Hela cells was detected quantitatively by ELISA;Changes in killing effects of lymphocytes on Hela cells expressing IL-12 were observed by MTT.It was found that Hela cells could express IL-12 between 24 h and 72 h after transfection.Killing activity of lymphocytes on Hela cells expressing IL-12 was significantly enhanced.It was concluded by cell transfection technique,Hela cells could express IL-12 and were more easily killed by lymphocytes.