高速逆流色谱法分离野马追乙酸乙酯萃取物中三种倍半萜烯内酯

摘要

A high-speed counter-current chromatography(HSCCC) method was established for the separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. n-butanol fraction. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:4:2:3) was selected. From 900 mg of n-butanol fraction of Eupatorium lindleyanum DC, 6.5mg of 3β-Hydroxy-8β- [4'-hydroxytigloyloxy ]-costunolide, 28.5 mg of eupalinolide A and 29.3 mg of eupalinolide B were obtained in one-step HSCCC separation, with the purity of 92.5%, 91.7% and 93.9%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H NMR.%建立了应用高速逆流色谱（HSCCC）从野马追乙酸乙酯萃取物中分离纯化3种倍半萜烯内酯的方法．采用正己烷-乙酸乙酯-甲醇-水（1：4：2：3）作为两相溶剂体系，从900mg野马追乙酸乙酯萃取物中分离得到313．Hydroxy-8β-[4＇-hydroxytigloyloxy]-costunolide6．5mg，野马追内酯A28．5mg和野马追内酯B29．3mg，其纯度分别为92．5％，91．7％和93．9％．3种化合物的结构通过ESI-MS和1HNMR进行了鉴定．