植物生长延缓剂多效唑和比久对怀山药离体保存的影响

摘要

In order to find the effective method for retarding growth of Dioscorea opposita Thunb. Germplasm resources in vitro, in this study, effects of PP333 and B9 on preservation of D. Op p. Thunb. Test-tube plantlets were studied using D. Op p. Thunb. Cv. Tiegun,47 and B as experimental materials. The results showed that at room temperature (25 °C ) or low temperature (4 °C ) , PP335 and B9 all could retard growth of Tiegun,47 and B test-tube plantlets,the preservation efficiency of PP333 was better than that of B9 , and the preservation efficiency of low temperature was better than that of room temperature. On the medium of MS + KT 2 mg/L + NAA 0. 02 mg/L under low temperature, the optimum mass concentration of PP333 for preservation of Tiegun,47 and B plantlets was 5. 0 mg/L,2. 5 mg/L and 1. 0 mg/L,respectively. Under the above condition,the test-tube plantlets could be preserved for 180 days without subculture; their survival rates were 100%, 100% and 95% ,respectively. In a word,the best method for retarding growth of D. Opp. Thunb. Germplasm resources in vitro was as follows: test-tube plantlets were cultured for 7 d on the MS+KT 2 mg/L+NAA 0. 02 mg/L added with the optimum PP333 at room temperature, and then transferred to low temperature and preserved under natural light.%为了寻找怀山药种质资源缓慢生长离体保存的有效方法,以铁棍山药、47号山药和B号山药为试材,研究了植物生长延缓剂多效唑(PP333)和比久(B9)对其试管苗在常温(25℃)和低温(4℃)下的保存效果.结果表明:常温和低温下,PP333、B9均可延缓各怀山药试管苗的生长,PP333的保存效果优于B9的,低温的保存效果优于常温的.在MS+KT 2 mg/L+NAA 0.02 mg/L培养基上,铁棍山药、47号山药和B号山药低温保存的适宜PP333质量浓度分别为5.0 mg/L、2.5 mg/L、1.0 mg/L,在此条件下,各怀山药无需继代可保存6个月,保存6个月的存活率分别为100％、100％、95％.在试验范围内,怀山药种质资源缓慢生长离体保存的有效方法为:将不同怀山药带芽节段接种在添加各自适宜质量浓度PP333的MS+KT 2 mg/L+NAA 0.02 mg/L培养基上,常温下培养7d,再转入4℃低温、自然光下保存.