紫茎泽兰乙醇提取物对五龄思茅松毛虫肠道细菌的抑制作用

摘要

以思茅松毛虫五龄幼虫肠道细菌为供试菌,采用牛津杯法测定紫茎泽兰不同部位乙醇提取物的抑菌效果.结果表明:紫茎泽兰不同部位乙醇提取物除茎提取物外,对5种思茅松毛虫肠道细菌(共10株)均有不同程度的抑制作用,抑制作用大小顺序为叶＞根＞茎.在相同质量浓度下,叶提取物对5种肠道细菌的抑菌作用大小顺序为雷金斯堡约克氏菌(Yokenella regensburgei)＞弗氏柠檬酸杆菌(Citrobacter freundii)＞产酸克雷伯氏杆菌(Klebsiella oxytoca)＞克雷伯氏杆菌(K lebsiella peneumoniae)＞苏云金芽孢杆菌(Bacillus thuringiensis),其对雷金斯堡约克氏菌、产酸克雷伯氏杆菌以及弗氏柠檬酸杆菌的最低抑菌质量浓度为0.125 g/mL,对其余2种细菌的最低抑菌质量浓度分别为0.25 g/mL、0.5 g/mL.根提取液对雷金斯堡约克氏菌和克雷伯氏杆菌无抑制作用,在相同质量浓度下,其对其余3种细菌的抑制作用大小顺序与叶提取液相同,最低抑菌质量浓度为0.25 g/mL.紫茎泽兰乙醇提取物具有良好的抑菌效果,可用于开发新型的植物源生物农药.%The Oxford cup method was used to measure the inhibition effects of ethanol extracts from different parts of Eupatorium adenophorum Spreng on intestinal bacteria from 5th instar larvae of Dendrolimu kikuchii. The results showed that except stems the ethanol extracts from leaves and roots of E. Adenophorum exhibited inhibition effects on 10 strains of intestinal bacteria and the effect order of different parts was leaves>roots>stems. Under the same concentration of leaf extracts, the order of inhibition effects on intestinal bacteria was Yokenella regensburgei^> Citrobacter freundii^> Klebsiella oxytoca^>Klebsiella peneumoniae~>Bacillus thuringiensis. The minimum inhibition concentration (MIC) was 0. 125 g/mL on Yokenella regensburgei,Klebsiella oxytoca and Citrobacter freundii ,0. 25 and 0. 5 g/mL on other 2 bacterial species. The root ex-tracts had no inhibition effects on Yokenella regensburgei and Klebsiella peneumoniae. Under the same concentration,the order of inhibition effects on other 3 bacterial species was consistent with that of leaf extracts,and the MIC of root extracts was 0. 25 g/mL. The ethanol extracts from E. Adenophorum had good inhibition effects on intestinal bacteria and could be used to develop new plant source bio-pesticide.