玉米单倍体诱导系EMK－1诱导率的测定和单倍体自然加倍技术研究

摘要

围绕黄淮海夏玉米区核心种质郑58、昌7－2改良的6类模式，以昌7－2／LX9801、昌7－2／78599、昌7－2／PH4CV、郑58／PH6WC、郑58／78599、郑58／K22的F1代为基础材料，探讨诱导系EMK－1在不同遗传背景下的诱导率，同时在河南省新乡地区进行单倍体的分期播种试验，研究当地生态条件对单倍体自然加倍的影响，为单倍体育种提供理论依据。结果表明，外引诱导系EMK－1对郑58种质的诱导率（平均8．08％）高于昌7－2（平均5．11％），对郑58／78599的F1代单倍体的诱导率最高（9．24％）。播期对单倍体自然加倍率的影响显著，在新乡地区3月30日—4月13日种植单倍体，自然加倍率较高。%Haploid inducing ratio of a foreign inducer EMK-1 for six hybrid combinations which were de-veloped by Chang 7-2/LX9801, Chang 7-2/78599, Chang 7-2/PH4CV, Zheng 58/PH6WC, Zheng 58/78599 and Zheng 58/K22 was determined in Huanghuaihai region,and haploid stage seeding experiments were set in Xinxiang,Henan province to study the effect of local ecological conditions on the natural dou-bling of haploids.The results showed that when the females were Zheng 58’ s improved lines,the average inducing ratio was 8.08%,if they were Chang 7-2’s improved lines,the number was 5.11%.The combi-nations from Zheng 58 and 78599 lines had the best inducing ratio of 9.24%.Sowing date had a signifi-cant effect on natural doubling effect of maize haploid,and It would be a better option for planting hap-loids between March 30th and April 13th,when the natural doubling rate was high.