为研究甘加藏羊发情周期血浆中生殖激素的分泌变化规律,选取24只处于发情周期内的甘加藏羊,采集甘加藏羊发情周期不同阶段的血液,采用酶联免疫分析法(ELISA)检测甘加藏羊发情周期血浆中促卵泡素(FSH)、促黄体素(LH)、孕酮(P)和雌激素(E)的分泌变化.结果显示,FSH含量在发情期最高,为3.406 mIU/mL,发情后期降低至2.492 mIU/mL,间情期又开始升高,至发情前期达2.727 mIU/mL,发情期含量极显著高于发情后期、间情期和发情前期;LH含量在间情期最高,为4.377 mIU/mL,发情后期显著低于间情期和发情前期;P含量在发情前期最高,为18.039 ng/mL,发情期降至16.196 ng/mL,发情后期缓慢升高,间情期达17.127 ng/mL,发情前期极显著高于发情期与发情后期;E含量在发情前期最低,为29.625 pg/mL,发情期升高(36.035 pg/mL),发情后期达到最高,为38.573 pg/mL,间情期又降低(35.531 pg/mL),发情后期极显著高于发情期、间情期以及发情前期,发情期与间情期极显著高于发情前期.%To study the secretion changes of reproductive hormones in Ganjia Tibetan sheep estrous cycle in plasma,the blood of different estrous cycle stages from 24 Ganjia Tibetan sheep was collected and the secretion dynamic change of follicle-stimulating hormone (FSH),luteinizing hormone (LH),progesterone (P) and estrogen (E) in plasma of the Tibetan sheep estrous cycle was tested by enzyme-linked immunoassay(ELISA).The results showed that content of FSH was the highest(3.406 mIU/mL)in the estrus,then reduced to 2.492 mIU/mL in the late estrus and began to rise in the dioestrus,and achieved 2.727 mIU/mL in the proestrus.The content of FSH in the estrus was significantly higher than that of the late estrus,dioestrus and proestrus.The content of LH in the dioestrus was the highest (4.377 mIU/mL),and the late estrus was significantly lower than dioestrus and proestrus.The content of P was the highest (18.039 ng/mL) in the proestrus,then reduced to 16.196 ng/mL in the estrus and rose slowly in the late estrus (achieved to 17.127 ng/mL in the dioestrus).The content of P in the proestrus was significantly higher than that of the estrus and the late estrus.The content of E was the lowest (29.625 pg/mL) in proestrus,rose to 36.035 pg/mL in the estrus and achieved to the highest in the late estrus(38.573 pg/mL),then reduced to 35.531 pg/mL in the dioestrus.The content of E in the late estrus was significantly higher than the estrus,dioestrus and proestrus,and that of proestrus was significantly lower than dioestrus and estrus.
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