麻疯树JcWRI1基因启动子克隆及功能分析

摘要

WRI1 plays an important role in controlling the conversion course from carbohydrates to lipids.We analyzed the expression pattern of the JcWRI1 in different tissues in Jatropha curcas L.by qRT-PCR.The result showed that JcWRI1 transcripts were predominantly expressed in seeds.On the other hand,the promoter of JcWRI1 was obtained from Jatropha curcas L.in this paper.Sequence analysis indicated that the promoter included TATA-box,CAAT-box,and a number of cis-acting elements involved in abscisic acid response,light response,stress response,endosperm-specific expression,respectively.The JcWRI1 promoter was fused with GUS reporter gene and transferred into Oryza sativa L..Histochemical staining of different organs of the transgenic plants showed that a very lowly GUS reporter gene expression in root,stem,leaf and higher expression in seed.The results indicated that the promoter functioned in the seed and regulated by multiple factors.%WRI1基因在控制从碳水化合物到脂类的转化过程中起着重要的作用.通过qRT-PCR方法分析了JcWRI1在麻疯树不同组织中的表达特性,结果表明:JcWRI1主要在种子中表达.同时克隆了麻疯树JcWRI1基因起始密码子上游2 000 bp序列.序列分析表明,该序列不仅包含有TATA-box和CAAT-box基本元件,而且含有胚乳表达所需的顺式调控元件、脱落酸应答相关顺式作用元件、光响应元件及各种胁迫响应元件.将该启动子与GUS报告基因连接构建表达载体,并转化水稻.GUS组织化学染色分析结果表明,JcWRI1启动子驱动GUS基因在根、茎、叶中有很低的表达,然而在种子中具有较高的表达活性.表明该启动子主要在种子中表达,并受多种因子的调控.