山羊FSHR基因编码蛋白的结构及功能分析

摘要

In this study,the physical and chemical properties,subcellular localization and function of goat FSHR gene were analyzed by ProtParam and ProtScale online software.Chou-Fasman and homology modeling methods were used to predict the secondary structure and tertiary structure,respectively.Phylogenetic trees of different species of FSHR genes were constructed by the neighbor joining method.Goat FSHR gene encoding protein was an unstable protein containing seven transmembrane domains and one G protein coupled receptors,and it had high hydrophilicity.The subcellular localization of FSHR gene was in endoplasmic reticulum,vacuole and mitochondria.Secondary structure was mainly alpha helix,and tertiary structure was rich in helix domain and long chain coil.Compared with other species,goats had closer genetic relationship with sheep,cattle and pig.Different mammalian FSHR genes were highly conserved in evolution,and phylogenetic results were consistent with their animal tayonomy.%通过ProtParam和ProtScale等在线软件对山羊FSHR基因编码蛋白的理化性质、亚细胞定位及功能进行分析,分别使用Chou-Fasman和同源建模方法对二级、三级结构进行预测,并对采用邻近法构建的不同物种FSHR基因编码蛋白的系统发育树进行分析.结果显示,山羊FSHR基因编码蛋白由695个氨基酸残基组成,包含7个跨膜域和1个G蛋白耦合受体,属于不稳定蛋白,具有较高的亲水性;亚细胞定位结果显示,FSHR基因编码蛋白分布在内质网、液泡和线粒体内;二级结构主要是α螺旋,三级结构富含helix螺旋结构域和长链卷曲.对不同物种FSHR基因编码蛋白进行系统发育分析发现,与其他物种相比,山羊与绵羊、家牛、野猪亲缘关系较近,系统发育分析结果与其动物学分类结果一致.