制备麦胚抗氧化多肽酶解条件优化的研究

摘要

A new optimization utilized path was researched by hydrolysis that it could enhanced the worth of wheat germ protein.Wheat germ protein were hydrolyzed by using alcalase,neutrase and papain.The best hydrolyzed parameter were determined by Degree of hydrolysis,protein solubility and Thiobarbituric Acid-Reactive Substances（TBARS value）with different enzyme,different hydrolyzed time,temperature and pH.Wheat germ protein hydrolysates possessed antioxidation,and the optimization enzyme was neutrase for 5 h.hydrolyzed temperature was 50 ℃,pH 7.0.The DH of neutrase-hydrolyzed wheat germ protein was 17.28%,TBARS value of wheat germ hydrolysates was 3.3 mL·L-1,and nonhydrolysis protein was 10.34 mL·L-1,so wheat germ hydrolysates possessed strong antioxidation.%为研究优化利用麦胚蛋白的新途径,以提高麦胚蛋白的附加值。采用碱性蛋白酶、中性蛋白酶和木瓜蛋白酶三种酶对麦胚蛋白进行水解,考察不同酶解产物、水解时间、水解温度和水解pH值条件下的水解度、蛋白溶解度和硫代巴比妥酸反应物值（TBARS）,确定制备麦胚抗氧化肽的较佳水解参数。研究结果表明：麦胚蛋白水解物具有抗氧化活性,而且制备麦胚抗氧化肽的最佳酶为中性蛋白酶,水解时间为5 h,最佳水解温度为50℃,pH值为7.0。水解度为17.28%时,麦胚肽的TBARS值为3.3 mL.L-1,未水解麦胚蛋白的TBARS值为10.34 mL.L-1,这就表明麦胚肽具有较强的抗氧化能力。