超顺磁性Fe2O3纳米粒子在3T3L1细胞内的代谢归宿

摘要

目的 观察Fe2O3磁性纳米粒子进入3T3L1细胞后的代谢归宿.方法 普鲁士蓝染色观察Fe2O3磁性纳米粒子能否被3T3L1细胞摄入,利用透射电子显微镜观察磁性纳米粒子在3T3L1细胞内的亚细胞分布情况.结果 Fe2O3磁性纳米粒子能够被3T3L1细胞摄入,且摄入量呈时间及剂量依赖性;进入3T3L1细胞内的Fe2O3磁性纳米粒子主要分布在细胞质的吞噬泡内,而在其他细胞器内未见分布;入胞的Fe2O3磁性纳米粒子可随细胞有丝分裂分配至子代细胞.结论 Fe2O3磁性纳米粒子进入3T3L1细胞呈时间及剂量依赖性,且入胞的磁性纳米粒子可以随着有丝分裂进入子代细胞,从而使细胞的纳米"载荷"逐渐下降.%Objective To investigate the metabolic fate of superparamagnetic iron oxide ( Fe2O3 ) nanoparticles ( SPION ) in 3T3L1 cells. Methods The internalization of SPION by 3T3L1 cells was observed by Prussian blue staining and the subcellular distribution of SPION in 3T3L1 cells was detected by transmission electron microscopy. Results SPION could be internalized by 3T3L1 cells; the internalized SPION were vesicle - bounded and mainly distributed in the cytoplasm. No SPION was found in other cell organelles. The internalized SPION could be redistributed into the offspring cells along with mitosis. Conclusion SPION can be internalized by 3T3L1 cells in time - and dose - dependent manners. The internalized SPION are redistributed to the offspring cells and therefore the nanoparticle burden of the cells is gradually reduced with cell division.