高效液相色谱法测定前胡中白花前胡甲素和白花前胡乙素的含量

摘要

Objective To improve the method for the determination of praeruptorin A and praeruptorin B in Peucedani Radix by high performance liquid chromatography.Then the method was used for the analysis of commercial “Qian Hu”.Methods Methanol was used as the extraction solvent and ultrasonic extraction time was 30 min.The extract was analyzed directly.The separation was performed on a Thermo ODS HYPERSIL(4.6× 250 mm,5 μm) column using methanol and water (68:32) as mobile phase.Results The methodology validation presented that praeruptorin A and praeruptorin B were in good correlation in the range of 5.00-400.00 mg/L and 0.50-40.00 mg/L with the regression equations of Y=28.478 222 4X+ 6.484 094 (r=0.999 99) and Y=25.589 318 7X-0.328 822 (r=0.999 99),respectively.The results of the precision (RSD) and recoveries were in the range of 0.6％-2.0％ and 90.7％-104.8％,respectively.Nine batches of commercial “Qian Hu” were determined by the established method.Conclusion The method is simpler,more rapid and more reproducible.It can be used for the accurate determination of praeruptorin A and praeruptorin B in Peucedani Radix.%目的 对前胡中白花前胡甲素和白花前胡乙素常用的高效液相色谱法含量测定方法进行改进,用于市售前胡的分析.方法 改用甲醇作为提取溶剂,超声处理时间为30 min,提取液直接测定分析.采用Thermo ODS HYPERSIL色谱柱(4.6×250 mm,5μm),以甲醇-水(68:32)为流动相.结果 白花前胡甲素和白花前胡乙素分别在5.00～400.00 mg/L和0.50～40.00 mg/L范围内线性关系良好,回归方程分别为Y=28.478 222 4X+6.484 094(r=0.999 99)和Y=25.589 318 7X-0.328 822(r=0.999 99),精密度为0.6％～2.0％,加样回收率为90.7％～104.8％.采用建立的方法对9批市售前胡进行了测定.结论 该方法更简便、快速、重复性好,可准确测定前胡中白花前胡甲素和白花前胡乙素.