Objective:To study the correlation of NOX1 and NOX2 expression in ulcerative colitis tissue with intestinal mucosal oxidative stress response and barrier function injury .Methods:69 patients who were diagnosed with ulcerative colitis in Yan'an People's Hospital between May 2015 and March 2017 were selected as the UC group of the research ,and 78 patients who were diagnosed with colon polyps were selected as the of polyps group of the research .The ulcerative colitis lesion and pol-yp lesion were collected to detect the expression of NOX1 and NOX2 ,the generation of oxygen free radicals as well as the con-tents of apoptosis molecules and mucosal barrier molecules .Results:The mRNA expression and protein expression of NOX1 and NOX2 in the intestinal mucosa of UC group were significantly higher than those of polyps group ;LPO ,MDA ,AOPP , NO ,PDCD5 and Bax levels in intestinal mucosa of UC group were significantly higher than those of polyps group and positive-ly correlated with the mRNA expression and protein expression of NOX1 and NOX2 while Bcl-2 ,Cdx1 ,Cdx2 ,galectin-1 ,ga-lectin-3 ,OCLN ,cingulin and ZO-1 levels were significantly lower than those of polyps group and negatively correlated with the mRNA expression and protein expression of NOX1 and NOX2 .Conclusion:The high expression of NOX1 and NOX2 in ulcera-tive colitis tissue can activate the intestinal mucosal oxidative stress response and result in the intestinal mucosal barrier func-tion injury .%目的:研究溃疡性结肠炎组织中NOX1、NOX2表达量与肠黏膜氧化应激反应、屏障功能损伤的相关性.方法:选择在本院诊断为溃疡性结肠炎的69例患者作为研究的U C组,选择诊断为结肠息肉的78例患者作为研究的息肉组.取溃疡性结肠炎病灶及息肉病灶,检测NOX1、NOX2的表达量以及氧自由基的生成量、凋亡分子和黏膜屏障分子的含量.结果:UC组肠黏膜内NOX1、NOX2的mRNA表达量和蛋白表达量均显著高于息肉组;UC组肠黏膜内LPO、MDA、AOPP、NO、PDCD5、Bax的含量均显著高于息肉组且与NOX1、NOX2的mRNA表达量和蛋白表达量呈正相关,Bcl-2、Cdx1、Cdx2、ga-lectin-1、galectin-3、OCLN、cingulin、ZO-1的含量均显著低于息肉组且与NOX1、NOX2的mRNA表达量和蛋白表达量呈负相关.结论:溃疡性结肠炎组织中N OX1、N OX2的高表达能够激活肠黏膜的氧化应激反应并造成屏障功能损伤.
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