喉癌组织中Six1、MTDH、TKTL1表达量与血清肿瘤标志物含量、细胞增殖活力的相关性

摘要

目的:探讨喉癌组织中Six1、MTDH、TKTL1表达量与血清肿瘤标志物含量、细胞增殖活力的相关性.方法:收集在本院确诊的原发性喉癌患者48例作为观察组,同期在本院进行喉镜检查的声带息肉患者50例作为对照组.采用荧光定量PCR法检测两组患者病灶组织中Six1、MTDH、TKTL1的表达量,癌基因及抑癌基因的表达量,检测血清中肿瘤标志物的含量.采用Pearson检测评估喉癌组织中Six1、MTDH、TKTL1表达量与血清肿瘤标志物含量、细胞增殖活力的相关性.结果:观察组病灶组织中Six1、MTDH、TKTL1、c-myc、STAT3、ras mRNA的表达量以及血清中肿瘤标志物CA72-4、CA19-9、CYFRA21-1、SCC-Ag、TK1的含量均高于对照组病灶组织,LKB1、CDKN2、PTEN mRNA的表达量低于对照组病灶组织;喉癌组织中Six1、MTDH、TKTL1表达量与血清CA72-4、CA19-9、CYFRA21-1、SCC-Ag、TK1的含量及c-myc、STAT3、ras的mRNA表达量呈正相关,与LKB1、CDKN2、PTEN的mRNA表达量呈负相关.结论:喉癌组织中Six1、MTDH、TKTL1表达量增高,且其具体表达量与肿瘤恶性程度直接相关.%Objective: To study the correlation of Six1, MTDH and TKTL1 expression in laryngeal cancer tissue with serum tumor marker levels and cell proliferation activity.Methods: A total of 48 patients who were diagnosed with primary laryngeal cancer in our hospital were enrolled as observation group, and 50 patients with vocal cord polyp who received laryngoscopy in our hospital during the same period were selected as control group.Fluorescence quantitative PCR was used to determine the expression of Six1, MTDH and TKTL1, and the expression of oncogenes and tumor suppressor genes in lesion tissue of two groups of patients, as well as the contents of tumor markers in serum.Pearson test was used to evaluate the correlation of Six1, MTDH and TKTL1 expression in laryngeal cancer tissue with serum tumor marker levels and cell proliferation activity.Results: Six1, MTDH, TKTL1, c-myc, STAT3 and ras mRNA expression in lesion tissue as well as tumor markers CA72-4, CA19-9, CYFRA21-1, SCC-Ag and TK1 levels in serum of observation group were higher than those of control group, while LKB1, CDKN2 and PTEN mRNA expression were lower than those of control group;Six1, MTDH and TKTL1 expression in laryngeal cancer tissue were positively correlated with serum CA72-4, CA19-9, CYFRA21-1, SCC-Ag and TK1 levels as well as c-myc, STAT3 and ras mRNA expression, and negatively correlated with LKB1, CDKN2 and PTEN mRNA expression.Conclusion: Six1, MTDH and TKTL1 expression increase in laryngeal cancer tissue, and their specific expression are directly correlated with tumor malignancy.