Effects of panax notoginseng saponins on apoptosis, inflammation and oxidative stress in rats with propofol-induced cognitive impairment

摘要

Objective: To study the effects of panax notoginseng saponins(PNS) on apoptosis, inflammation and oxidative stress in rats with propofol-induced cognitive impairment. Methods: 7-day-old SD rats were chosen as experimental animals and randomly divided into control group, propofol group and PNS intervention group, propofol group were given intraperitoneal injection of propofol, and PNS intervention group were given panax notoginseng saponins intervention on the basis of intraperitoneal injection of propofol. The brain tissues of three groups of rats were collected 7 d after modeling and intervention to measure the expression of apoptotic molecules, inflammatory molecules and oxidative stress molecules. Results: Brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), Bax inhibitor 1 (BI-1), DNA dioxygenase (TET2), peroxidoreductin 6 (PRDX6), heat shock protein 70 (HSP70) mRNA expression in brain tissues of propofol group were lower than those of control group whereas Bcl-2 related X protein (Bax), glycoprotein 78 (GRP78), caspase-3 containing cysteine, nuclear factor-κB (NF-κB), interleukin-1β(IL-1β), tumor necrosis factor-a(TNF-a), neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), NADPH oxidase 2 (NOX2), malondialdehyde (MDA) mRNA expression were higher than those of control group; BDNF, NGF, BI-1, TET2, PRDX6 and HSP70 mRNA expression in brain tissues of PNS intervention group were higher than those of propofol group whereas Bax, GRP78, Caspase-3, NF-κB, IL-1β, TNF-α, nNOS, iNOS, NOX2 and MDA mRNA expression were lower than those of propofol group. Conclusion: Panax notoginseng saponins can reduce the apoptosis, inflammation and oxidative stress in rats with propofol-induced cognitive impairment.