Correlation of miR-29b expression level with oxidative stress mediators and inflammatory cytokines in diabetic foot wound

摘要

Objective:To study the correlation of miR-29b expression level with oxidative stress mediators and inflammatory cytokines in diabetic foot (DF) wound.Methods: The DF patients admitted to our hospital between February 2015 and October 2017 were selected as the DF group and wound tissues were collected; non-diabetic patients admitted to our hospital due to trauma during the same period were selected as the control group and normal skin tissues were collected. The expression level of miR-29b as well as the contents of oxidative stress mediators and inflammatory cytokines in the tissues was measured.Results: miR-29b expression as well as malondialdehyde (MDA), interleukin-1β (IL-1β), interleukin-18 (IL-18), tumor necrosis factor-α (TNF-α), vascular cell adhesion molecule-1 (VCAM-1) contents in the wounds of DF group was significantly higher than those of control group while superoxide dismutase (SOD) and glutathione peroxidase (GPX) contents were significantly lower than those of control group; miR-29b expression was positively correlated with MDA, IL-1β, IL-18, TNF-α and VCAM-1 contents, and negatively correlated with GPX and SOD contents. Conclusion: The high expression of miR-29b in diabetic foot wound is related to the over-activation of oxidative stress response and inflammatory response.