Studies on hemolysis properties of medicalα-calcium sulfate hemihydrate

摘要

Objective:The as-providedα-calcium sulfate hemihydrate bone repair material exhibits superior degradation properties and osteogenic property to the traditional calcium sulfate bone repair materials. Its effects on the blood and the biocompatibility were studied to ensure its safety for the bone implantation application.Method: The hemolytic tests were conducted to evaluate the blood compatibility of theα-calcium sulfate hemihydrate. The extracts ofα-calcium sulfate hemihydrate with different concentration reacted with 2% rabbit red blood cell suspension. The existence and morphology of the red blood were observed through naked eye observation and microscope observation. The hemolytic ratios were calculated through the absorbances using a ultraviolet spectrophotometer.Result:It was observed by naked eye that the red blood accumulated at the bottom of the test tubes and the upper liquids were clear, colorless and transparent in the experimental sample. The microscope observation exhibited that the cells of the experimental samples had the normal morphology, no deformed and broken blood sell existed. The hemolytic ratios of the experimental sample were all less than 5%. The hemolytic ratios were increased with the increase of the concentration of the extracts. Conclusions: The hemolytic ratios of the medical -calcium sulfate hemihydrate accorded with the standard ISO/TR7405-1984(E). It could be concluded the material would not cause acute hemolysis. The osteogenic property exhibited dose dependence.