Difference of laser-induced autofluorescence spectra of mladder cancer lesion beforetissue

摘要

Objective:To analyze the characteristics and difference of the laser-induced autofluorescence spectra of normal bladder tissue and bladder carcinoma tissue and find a method for discrimination among normal bladder tissue and bladder carcinoma tissue.Methods:Laser-induced autofluorescence detection analytical system was adopted to collect 47 autofluorescence spectra of cutting normal bladder tissue and bladder carcinoma tissue in surgery in 300-800 nm. A total of 25 bladder specimens of rats were involved to detecte collagen Ⅳ and cathepsin B expression in each group: normal bladder tissue and bladder carcinoma tissue from the rat colorectal carcinoma model. The collagen Ⅳ and cathepsin B was detected with immunohistochemical staining. The correlation of the results was studied. Cathepsin B RNA expression was detected with reverse transcription-polymerase chain reaction (R-TPCR).Results: Of 94 specimens detected by laser-induced autofluorescence, 47 were normal and 47 carcinoma tissues. All normal tissue fluorescence intensity was high than carcinoma tissue on the same wave length. There was a char wave crest at 452 nm respectively. Normal tissue of peak intensity was obviously high than carcinoma tissue (P<0.05). Collagen Ⅳ expression existed in all specimens in different degrees, it was decreased carcinoma group, and there was significant difference compared with the normal group and hyperplasia group. The expression of cathepsin B in carcinoma group was increased compared with normal group (P<0.05).Conclusions: Normal tissue and carcinoma tissue of autofluorescence intensity are different, laser-induced bladder tissue autofluorescence spectra may be used in distinguishing Normal tissue and carcinoma tissue. The expression of collagen Ⅳ is decreased in bladder carcinoma compared with the normal bladder tissue. The expression of cathepsin B is increased in bladder carcinoma compared with the normal bladder tissue, and possiblely induces difference of one of reason for autofluorescence in normal bladder tissue and bladder carcinoma tissue.