Value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer

摘要

Objective: To study the value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer. Methods: Patients with cervical cancer who underwent surgical resection in Yan'an University Affiliated Hospital between March 2015 and May 2017 were selected as cervical cancer group of the research, and patients with benign lesions who underwent hysterectomy in Yan'an University Affiliated Hospital during the same period were selected as the control group of the research. Before operation, cervical exfoliated cytology was taken for DNA ploid detection; before operation, the cervical tissue was taken to determine the expression of proliferation genes and invasion genes. Results: The number of exfoliated cells with abnormal DNA ploid in cervical cancer group was significantly higher than that in control group; Id-1, Ki-67, TET1, S6K1, CatL and ILK mRNA expression in cervical cancer tissue of cervical cancer group were significantly higher than those of control group whereas HSG, MCPH1, p16, TIMP1 and TIMP2 mRNA expression were significantly lower than those of control group; Id-1, Ki-67, TET1, S6K1, CatL and ILK mRNA expression in cervical cancer tissue with 3 exfoliated cells with abnormal DNA ploid were significantly higher than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid whereas HSG, MCPH1, p16, TIMP1 and TIMP2 mRNA expression were significantly lower than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid. Conclusion: DNA ploid detection can be used to evaluate the proliferation and invasion of cancer cells in cervical cancer.