贵阳市常见蚊虫共生菌沃尔巴克体的初步调查

摘要

Objective:To investigate the infection status and genotype of symbiotic bacteria (Wolbachia) in common mosquito in Guiyang.Methods:Mosquitoes were collected by using a spoon from 6 districts of Guiyang (Yunyan,Nanming,Huaxi,Xiuwen,Xifeng and Kaiyang) and identified by morphology in the lab.Wolbachia surface protein gene (wsp) was amplified by PCR and sequenced for phylogenetic analysis.Results:There were 3 species mosquito found in the different habitats in the 6 districts,Aedes albopictus,Culex pipens pallens and Armigeres obturbans.The positive rate of wsp ranged from 42.9％ to 95％.13 target gene sequences were obtained with sequence length between 572 to 602bp.Based on the phylogenetic tree,these sequences were divided into A and B supergroup respectively.The wolbachia genotype of A albopictus was wAlbA and wAlbB.The Wolbachia genotype of Culex pipens pallens was wPip.The Wolbachia genotype of Armigeres obturbans was wAlbA.Conclusions:Aedes albopictus,Culex pipens pallens and Armigeres obturbans in Guiyang are generally infected with Wolbachia and their genotypes are wAlbA,wAlbB and wPip.%目的:了解贵阳市常见蚊虫种群中沃尔巴克体(Wolbachia)的感染情况及基因型.方法:采用勺舀法采集贵阳市6城区(云岩区、南明区、花溪区、修文县、息烽县和开阳县)的蚊虫并鉴定;选用Wolbachia表面蛋白基因(wsp),利用PCR方法对单只雌蚊进行扩增、测序并做系统发育分析.结果:在贵阳市6城区不同水体采集到的常见蚊种有3种,分别是白纹伊蚊、致倦库蚊和骚扰阿蚊,3种蚊虫种群Wolbachia的核酸阳性率为42.9％～95.0％;获得13条目的基因序列、序列长度为572 ～ 602 bp,系统发育树显示这些序列分别属于A和B两个超群,白纹伊蚊Wolbachia基因型为wAlbA和wAlbB,致倦库蚊Wolbachia基因型为wPip,骚扰阿蚊Wolbachia基因型为wAlbA.结论:贵阳市白纹伊蚊、致倦库蚊和骚扰阿蚊普遍感染Wolbachia,其基因型为wAlbA、wAlbB和wPip.