四君子汤总多糖对化疗荷瘤小鼠肠黏膜和整体免疫损伤的保护作用

摘要

[目的]研究四君子汤总多糖对化疗所致荷瘤小鼠肠黏膜和整体免疫损伤的保护作用.[方法]通过胸腺指数、脾脏指数、外周血淋巴细胞数目、抑瘤率及脾脏淋巴细胞分泌白细胞介素-2(IL-2)等指标观察四君子汤总多糖对化疗荷瘤小鼠整体免疫功能的影响；通过肠系膜淋巴细胞IL-2及肠道分泌型IgA(sIgA)的分泌量观察其对化疗荷瘤小鼠肠道免疫功能的影响.[结果]四君子汤总多糖能促进肠系膜淋巴细胞分泌IL-2,增加肠道sIgA的分泌量,对化疗荷瘤小鼠所致胸腺指数、脾脏指数、外周血淋巴细胞数目降低表现出恢复作用,并能促进脾脏淋巴细胞分泌IL-2(均P＜0.05或P＜0.01).[结论]四君子汤总多糖可通过调节化疗荷瘤小鼠肠道黏膜免疫和整体免疫功能,改善化疗对荷瘤小鼠所致的免疫损伤.%Objective To study the protective effect of polysaccharides of Sijunzi Decoction (SD) on the intestinal mucosa and systematic immune injury of tumor-bearing mice after chemotherapy. Methods S180 cells were inocubated into BALB/c mice to induce solid tumor, and then the model mice were randomized into model group, cyclophosphamide group ( intravenous injection of cyclophosphamide 20 mg/kg) , and SD polysaccharides group (oral use of SD polysaccharides 20 mg/kg, and intravenous injection of cyclophosphamide 20 mg/kg). Normal control group was also set up. Effect of SD polysaccharides on the whole immunity was investigated through the observation of thymus index, spleen index, peripheral blood lymphocyte count, tumor suppression rate and interleukin 2 (IL-2) level in splenic lymphocytes of tumor-bearing mice. Moreover, intestinal slgA level and IL-2 level in the mesenteric lymph nodes ( MLNs) were detected for the evaluation of the effect on intestinal mucosal immunity in tumor-bearing mice. Results SD polysaccharides obviously promoted the secretion of IL-2 level in MLNs and intestinal slgA level, up-regulated the decreased thymus index, spleen index and peripheral blood lymphocyte number, and promoted the secretion of IL-2 in splenic lymphocytes in tumor-bearing mice after chemotherapy (P <0. 05 or P < 0. 01). Conclusion SD polysaccharides can relieve immune injury induced by cyclophosphamide through regulating the intestinal mucosal immunity as well as the systematic immunity in tumor-bearing mice after chemotherapy.