Objective:To investigate the expressions and clinical significance of Th1/Th2 cytokines in peripheral blood of endometrial carcinoma patients.Methods:The expression of Th1 cytokines interleukin-2 (IL-2),interferon-γ (IFN-γ) and tumor necrosis factor-β (TNF-β) and Th2 cytokines (IL-4,IL-6 and IL-10) in peripheral blood of 35 cases of endometrial carcinoma patients before and after operation and 35 cases of healthy female were analyzed by fluorescent quantitative PCR (qPCR).Results:Before operation,compared with the healthy controls,the expression of Th2 cytokines(IL-4 and IL-6)in endometrial carcinoma increased,and the expression of Th1 cytokines (IFN-γ and TNF-β) declined and the Th1/Th2 ratio decreased significantly (P ＜0.05 or P ＜0.01).The Th1/Th2 ratio was negatively correlated with endometrial carcinoma stage.In middle-late stage patients,the Th1/Th2 ratio was statistically different from those of the early stages patients.But there was no significant correlation between the Th1/Th2 ratio and the pathological types or classification.After surgery,IL-4 was decreased significantly (P ＜0.01),while IFN-γ,TNF-β and Th1/Th2 ratio were increased in endometrial carcinoma group(P ＜0.05).Th1/Th2 ratio in the endometrial carcinoma after operation was not significantly different from that in the normal control groups (P ＞0.05).Conclusion:Th1/Th2 was imbalance in endometrial carcinoma,Th1/Th2 ratio inclined to Th2 may be the reason for occurrence and development of endometrial cancer.Surgery may help to maintain the Th1/Th2 balance.%目的:探讨外周血Th1和Th2细胞因子在子宫内膜癌患者中的表达及临床意义.方法:采用实时荧光定量PCR(qPCR)法检测35例子宫内膜癌患者(研究组)及35例正常女性(对照组)外周血中Th1型细胞因子白细胞介素(IL)-2、干扰素-γ(IFN-γ)、肿瘤坏死因子-β(TNF-β)以及Th2型细胞因子IL-4、IL-6及IL-10的表达水平,并分析Th1/Th2比值与患者病理类型、临床分期的关系.结果:与对照组比较,研究组患者术前Th2型细胞因子IL-4、IL-6基因相对表达量升高,Th1型细胞因子IFN γ、TNF-β基因相对表达量降低,Th1/Th2比值显著降低(P＜0.05或P＜0.01).子宫内膜癌中晚期较早期患者Th1/Th2比值降低,差异有统计学意义(P＜0.05),而不同子宫内膜癌病理类型及分级的Th1/Th2比值比较差异无统计学意义(P＞0.05).研究组患者术后Th1型细胞因子(INF-α、TNF-β)较术前升高,Th2型细胞因子(IL-4)较术前降低,Th1/Th2比值升高,差异均有统计学意义(P ＜0.05或P＜0.01),研究组术后与对照组Th1/Th2比值比较差异无统计学意义(P＞0.05).结论:Th1/Th2平衡向Th2方向偏移可能是子宫内膜癌发生发展的原因之一,手术治疗可以维持子宫内膜癌患者Th1/Th2平衡状态.
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