Objective To establish a new approach using HPLC with a monolithic column for determination of 8 saponins in Notoginseng and its preparation simultaneously. Methods HPLC was used with Merck Chromolith Performance RP-18e ( 100 mm× 4.6 mm, macropore 2 μm, mesopore 13 nm) . The mobile phase A and B were acetonitrile and water with gradient elution ( 0 ～ 20 min, 18％A; 20 ～ 30 min, 18％ ～30％A; 30～45 min, 30％A; 45～48 min, 30％ ～38％A; 48～57 min, 38％ ～18％A) , and the flow rate was 1.0 mL/ min. The injection volume was 10 μL, the detection wavelength was set at 203 nm, and the column temperature was at 30 ℃ . Results Ginsenoside Rg1 , Re, Ro, Rb1 , Rb3 , Rd, 20( S) -F1 and notoginsenoside R1 in Panax Notoginseng and its preparation were separated at baseline with good linearity within the range of 0.06～21.50 μg ( r = 0.9996) , 0.09 ～ 36.08 μg ( r = 0.9996) , 0.05 ～ 20.56 μg ( r= 0.9996) , 0.02 ～4.08 μg( r= 0.9996) , 0.04 ～ 16.60 μg ( r = 0.9995) , 0.03 ～ 8.90 μg ( r = 0.9994) , 0.02～ 6.22 μg ( r =0.9998) , and 0.03～12.58 μg ( r= 0.9996) , with a detection limit of 16.2, 27.1, 15.4, 3.06, 12.4, 6.7, 4.6, and 9.4 ng, respectively. Conclusion The method is simple, accurate and well separated, which could be used for determination of 5 saponins in Notoginseng and its preparation.%目的 建立同时测定三七及其制剂中8种不同极性的皂苷质量分数的HPLC法.方法 色谱柱:Merck Chromolith Performance RP-18e(100 mm×4.6 mm,macropore 2μm,mesopore 13 nm);流动相:流动相A为乙腈,流动相B为水,梯度洗脱(0～20 min,18％A;20～30 min,18％ ～30％A;30～45 min,30％A;45～48 min,30％ ～38％A;48～57 min,38％ ～18％A);流速:1.0 mL/min;进样量:10μL:检测波长:203 nm;柱温:30℃.结果 三七皂苷R1和人参皂苷Rg1、Re、Ro、Rb1、Rb3、Rd、20(S)-F18种皂苷分离度良好,线性范围分别为0.06～21.50μg(r=0.9996)、0.09～36.08μg(r=0.9996)、0.05～20.56μg(r=0.9996)、0.02～4.08μg(r=0.9996)、0.04～16.60μg(r=0.9995)、0.03～8.90μg(r=0.9994)、0.02～6.22μg(r=0.9998)、0.03～12.58μg(r=0.9996),三七皂苷R1和人参皂苷Rg1、Re、Ro、Rb1、Rb3、Rd、20(S)-F1的检测限分别为16.2、27.1、15.4、3.06、12.4、6.7、4.6、9.4 ng.结论 所建方法简便、准确、分离效果好、无干扰,可同时分离三七及其制剂中三七皂苷R1和人参皂苷Rg1、Re、Ro、Rb1、Rd、Rb3、20(S)-F18种皂苷,其中5种皂苷成分可以定量.
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