LB膜法固定化蛋白质及其在生物传感器中的应用

摘要

LB（Langmuir-Blodgett）膜技术可以在分子水平上控制膜厚及分子取向,是实现新型功能材料设计和开发的重要手段。将具有生物功能的蛋白质引入LB膜材料,在生物传感器、仿生膜及生物催化等领域具有广阔的应用前景。本文主要对近几年关于LB膜法固定化蛋白质的方法、影响因素以及在生物传感器方面的应用进行总结,并展望了相关的研究应用前景。%LB（Langmuir- Blodgett） technique is an advanced technique to design novel functional materials, because it can control thickness and well-ordered films in molecular level. Biofunctional protein immobilized with LB films can be widely applied in biosensor, bionic membrane and biocatalysis areas. The methods of immobili- zing, influence factors and application in biosensors of protein LB films are summarized in this review.