丝素/明胶三维多孔支架的构建及其结构和性能表征

摘要

应用冷冻干燥技术,在不同预冻温度下制备不同质量比的三维多孔支架,用扫描电镜观察支架的微观形貌,X射线衍射仪分析其表面相结构,并测定了其吸水率、孔隙率和热稳定性,观察不同质量比和预冻温度对丝素(silk fibroin,SF)/明胶(gelatin,G)三维多孔支架结构和性能的影响。结果显示,通过改变材料的质量比和预冻温度可以控制支架的孔隙形貌、吸水率和孔隙率,并且将丝素与明胶复合可以使支架的热稳定性提高,同时结构更加稳定。%In this study,different mass ratio SF/G three dimensional porous composite scaffolds were made by freezing-dry method in different freezing temperature to explore the effect of mass ratio and freezing tempera-ture on pore structure and performance of three dimensional porous silk fibroin/gelatin composite scaffolds.The morphology of the SF/G scaffolds was observed under a scanning electron microscope;the structure of the scaf-folds was evaluated by X-ray diffraction;the water absorption,porosity and thermostability were also calculated. The result showed that the pore structure,water absorption and porosity of the scaffolds could be controlled by adj usting the mass ratio of material and freezing temperature,and more stable scaffolds with higher thermosta-bility could be created with the method of blending and modifying silk fibroin and gelatin.