兔Vx2肝癌改良模型的建立及其DSA影像分析

摘要

AIM　To establish suitable metastatic rabbit Vx2 liver tumor model for experimental study, probe into different tumor transplanted methods and analyze the DSA (digital substract angiography) imagining features of the liver tumor. METHODS　60 male New Zealand white rabbits were divided into 3 groups at random, 20 in each group. 5×107 Vx2 carcinoma cells were injected via hepatic artery, pecutaneous into liver by syringe needle in the different 2 groups (control groups); the 3rd group (retrofit group) were transplanted tumor tissue mass (about 106～108 carcinoma cells) into liver. Then we observed: ①the success rate of implanting tumor; ②the volume change of tumor by ultrasonograpy (the growth rate of tumor was calculated); ③the biological features of tumor (histopathology and electronic microscope photographs); ④the DSA imagining features of tumor. RESULTS　The success rates of transplanting tumor of 3 groups were 7/20, 10/20, 19/20; the retrofit group was the highest of 3 groups (P<0.05). The tumor grew as exponential curve. Histopathology and electronic microscope photographs indicated that tumor grew in the liver tissue and infiltrated into the normal liver tissue, and that it had the similar biological features of squamous cell carcinoma transplanted in other sites of rabbits. The DSA imagining of tumor indicated that the carcinoma had liberal blood vessels. CONCLUSION　A retrofit metastatic rabbit Vx2 liver carcinoma model has successfully been set up, The success rate of the method by which tumor tissue mass transplanted is obviously higher than the other two methods. This makes it possible to gain a reliable mature large tumor animal model for study.%目的　建立可供实验研究的稳定的兔Vx2移植性肝癌模型，探讨不同植瘤方式的成功率，并分析该肿瘤的DSA影像特征. 方法　新西兰白兔60只，随机分3组，每组20只. 将Vx2瘤细胞（5×107个）经肝动脉或经肝包膜分别接种于2组兔的肝左叶，建立对照肝癌模型. 第3组经肝包膜植入瘤组织块（约含106～108个瘤细胞）建立改良肝癌模型. 观察：①不同组植瘤的成活率；②改良组肿瘤7，10，14，17和21 d时的体积（B超测），并计算肿瘤生长率；③大体及镜下(光镜和电镜)瘤组织形态特征；④改良Vx2移植性肝癌的DSA影像特征. 结果　3组植瘤成活率分别为7/20，10/20和19/20，改良组植瘤成活率最高（P<0.05)，瘤体呈指数性生长，组织病理及电镜表明该瘤在肝组织中浸润式生长，其形状与移植于兔其他部位的Vx2鳞状细胞癌特征相似. DSA影像示该移植性肝癌具有丰富的血供. 结论　成功建立了兔Vx2移植性肝癌改良模型，瘤组织块种植方式成功率明显高于动脉途径和细胞液注射方式，为肝癌研究提供了大型实验模型.