大鼠肌肉挫伤后COX6C mRNA的表达与损伤时间关系

摘要

目的：探讨肌肉挫伤后组织中细胞色素c氧化酶亚基Ⅵc（cytochrome c oxidase subunit Ⅵc，COX6C） mRNA的表达变化及其与损伤时间的关系。方法54只健康成年SD大鼠随机被分为正常对照组和损伤后0.5、1、6、12、18、24、30和36 h组，采用重力锤自由落体方式制作大鼠肌肉挫伤模型。于相应时间提取挫伤处肌肉组织样本进行常规组织学观察，并提取组织中总RNA采用real-time PCR检测COX6C mRNA相对表达量。结果损伤后6h内仅见肌纤维间出血、肌细胞肿胀等改变，未见炎症细胞浸润。损伤6h以后逐渐出现肌细胞变性、坏死、炎症细胞浸润、纤维结缔组织增生等改变。COX6C mRNA在损伤后6 h以前其表达量均高于正常对照组，而在损伤6~36 h，其表达量均低于正常对照组。结论 COX6C mRNA在大鼠肌肉挫伤后呈现规律性表达，结合组织学检查有助于损伤时间的推断和研究。%Objective T o investigate the relation betw een injury tim e and the expression of cytochrom e c oxidase subunitⅥc (COX6C) m R N A in skeletal m uscle of rat after contusion. Methods A total of fifty-four SD rats w ere divided into the control group and the contusion groups (0.5,1,6,12,18, 24, 30, and 36 h after contusion), random ly. T he contusion m odel w as established by free fall drop of gravity ham-m er. A t corresponding tim e point after contusion, the regular histology w as exam ined and expression level of COX6C m R N A w as tested by real-tim e PC R after extraction of total R N A from the tissues. Results T he m ain pathological features of 6 h after injury included edem a and hem orrhage in m yocytes w ith no inflam m atory cells found. A fter 6 hours, the findings included m yocyte degeneration and necro-sis, inflam m atory cells infiltration, and fibrous connective tissue proliferation in the contused zone. T he expression level of COX6C m R N A w as higher than that of the control group w ithin 6 h after contusion. T he expression level w as low er than that of the control group from 6-36 h after contusion. Conclusion T he level of COX6C m R N A expresses in a regular w ay after contusion. It m ay be useful for estim ating w ound age in com bination w ith the results of pathological features.