解吸附电晕束电离质谱法快速检测功能保健食品中违禁添加的降糖类药物

摘要

目的建立解吸附电晕束电离质谱快速检测保健食品中违禁添加的6种降糖类化学合成药物(那格列奈,二甲双胍,伏格列波糖,格列吡嗪,吡格列酮,瑞格列奈)的分析方法。方法通过DCBI-MS直接分析,对比标准物质一级和二级质谱图,对检测样品中非法添加的那格列奈、二甲双胍、伏格列波糖、格列吡嗪、吡格列酮、瑞格列奈等6种降糖类化学合成药物进行快速定性和半定量检测。结果各目标物在一定范围内线性关系良好,线性相关系数R2均大于0.98,检测限均在1 mg/L以下,相对标准偏差为8%∼40%。结论此方法可以完成降糖类违禁药物的快速检测,定性准确,专属性高,同时完成半定量分析,为进一步准确测定样品提供依据。%　　Obiective To develop a rapid method for detection of 6 kinds of sugar-reducing medicines (na-teglinide, dimethyldiguanide, voglibose, glipizide, pioglitazone, repaglinide) with the desorption corona beam io-nization mass spectrometry (DCBI-MS) technique. Methods The rapid qualitative and semi-quantitative identi-fication of 6 kinds of sugar-reducing medicines including nateglinide, dimethyldiguanide, voglibose, glipizide, pioglitazone and repaglinide in samples were performed by comparing MS and MS2 spectra of samples with stan-dard substances by DCBI-MS. Results The method showed a good linearity in a given range for all of analytes. The linear coefficients of analytes were higher than 0.98. The LODs of 6 sugar-reducing medicines were lower than 1 mg/L. The RSDs of the method ranges from 8%to 40%. Conclusion The developed DCBI-MS is simple, rapid, sensitive for screening and semi-quantification of the sugar-reducing medicines in health foods and tradi-tional Chinese patent medicines and it provides the basis for further accurate regulation test.