杯碟法和酶热仪法检测乳及乳制品中β-内酰胺酶类物质的比较

摘要

本文对β-内酰胺酶的多种国内外检测方法进行了介绍，并着重比较了杯碟法和酶热仪法的特点，详细分析了两种方法在检测原理、仪器设备、实验操作、实验周期、结果判定、方法学参数等方面的异同点。杯碟法稳定可靠、灵敏度高，是目前中国乳及乳制品中β-内酰胺酶检测的推荐方法；酶热仪法简便快捷、可实现半定量检测，更适合于大规模样品筛查和现场检测。两种方法的测定结果具有可比性，可根据检测要求和实验室情况配合使用。%An overview of the determination methods forβ-lactamase in milk and dairy products was pre-sented, with discussion of the advantages and disadvantages of cylinder plate and enzyme thermistor biosensor method in detail. The advantages of reliability and sensitivity had been made the cylinder plate method a rec-ommended strategy forβ-lactamase test in China. Compared to the conventional culture-based assay, enzyme thermistor biosensor provided a rapid, simple and semi-quantification tool for routine use and on-site inspection. Both of the two methods were competent forβ-lactamase analysis in milk and dairy products, showing results in a good agreement.