高效液相色谱法测定角鲨烯软胶囊中的角鲨烯

摘要

Objective To establish a method for the determination of squalene in squalene soft capsules by high performance liquid chromatography. Methods Samples were extracted with methanol, and being separated by waters spherisorb C18 column with the mobile phase of methanol/acetonitrile (V:V=60:40), and the flow rate was 1.2 mL/min, the column temperature was 40 ℃, the content of squalene were determined by HPLC under the detection wavelength at 203 nm. Results The method showed a good linearity in the range of 0.1023~0.5118 mg/mL for squalene with the coefficient r>0.999, the detection limit was 0.0025%, the average recovery of different adding levels was 100.5%, and the relative standard deviation was 0.6%(n=9). Compared with the WS-1001-(HD-080-18)-2002 squalene gas chromatography method of National Drug Standards, there was no statistical difference between the two results. Conclusion HPLC method is more quick and easy, saving testing cost, and applicable to measure the content of squalene in health foods.%目的：建立高效液相色谱法测定角鲨烯软胶囊中角鲨烯的含量。方法样品经甲醇提取,以甲醇/乙腈(V：V=60：40)为流动相,流速为1.2 mL/min,经高效液相色谱在色谱柱Waters Spherisorb C18分离,柱温为40℃；然后在检测波长为203 nm的条件下测定角鲨烯的含量。结果角鲨烯的浓度在0.1023~0.5118 mg/mL的范围内与峰面积线性关系良好,相关系数r>0.999,方法的检出限为0.0025%,在不同添加水平下,方法的平均回收率为100.5%,相对标准偏差为0.6%(n=9)。与我国《国家药品标准》中WS-1001-(HD-080-18)-2002角鲨烯气相色谱法测定比较,两种结果无显著差异。结论高效液相色谱法更快捷简单,节省检测成本,可应用于保健食品中角鲨烯的含量测定。