高效液相色谱-质谱联用法检测鱼油和南极磷虾油中氧化三甲胺

摘要

Objective To establish a method for determination of trimethylamine-N-oxide (TMAO) in fish oil and Antarctic krill oil by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and determine the TMAO content in 5 types of fish oils and 7 kinds of Antarctic krill oils.MethodsFish oils and Antarctic krill oils were dissolved by diethyl ether, and then TMAO was extracted by normal saline and sodium hydroxide solution respectively. The content of TMAO was determined by multiple-reaction monitoring (MRM) mode after ultra-filtration. TMAO content in various fish oils and Antarctic krill oils were determined by the established method.Results TMAO had a good linearity in range of 5~250μg/mL. The recoveries of TMAO in fish oil and Antarctic krill oil were 83.5%~111.8%. TMAO was not detected in commercial fish oil. TMAO content among Antarctic krill oil made with different extracting techniques were different.Conclusion The established method for TMAO detection is rapid, accurate and sensitive, which is suitable for monitoring of TMAO content in fish oil and Antarctic krill oil.%目的 建立高效液相色谱-质谱联用法(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)测定鱼油和南极磷虾油中氧化三甲胺(trimethylamine-N-oxide,TMAO)含量的定量分析方法,并对5种不同类型的鱼油和6种南极磷虾油中TMAO的含量进行测定.方法 用乙醚作为鱼油和南极磷虾油的溶剂,以生理盐水和氢氧化钠溶液分别萃取鱼油和南极磷虾油中的TMAO,超滤离心后通过HPLC-MS/MS的多反应监测模式(MRM)检测TMAO;利用所建立的方法测定不同类型的鱼油和不同加工工艺的南极磷虾油中TMAO的含量.结果 TMAO在5~250μg/mL范围内线性关系良好,鱼油及南极磷虾油中TMAO回收率在83.5%~111.8%之间.市售鱼油中未检测到TMAO的存在.不同加工工艺制备的南极磷虾油中TMAO含量存在差异.结论 所建立的HPLC-MS/MS测定TMAO的方法快速准确,灵敏度高,可用于鱼油及南极磷虾油中TMAO含量的分析测定.