大猿叶虫滞育品系和非滞育品系交配行为的比较

摘要

The cabbage beetle Colaphellus bowringi Baly (Coleoptera:Chrysomelidae) is a serious pest of crucifers and goes into summer or winter diapause as adults.Compared to the diapause strain (DS),the reduced fecundity of non-diapause strain (NDS) is possibly related to mating behavior.The NDS of C.bowringi is a promising model for investigating the mechanism of diapause inheritance and regulation.In this study,we observed the mating rhythm,mating duration and mating frequency of the DS and NDS in C.bowringi at 25C and L:D =12:12 h.The results showed that mating rhythm of both strains peaked at 2 h after light period began,and the mating rate of NDS was significant lower than that of DS.The mating durations of the first time of DS and NDS were 122.7 ± 7.4 min and 100.1 ± 10.2 min,respectively,and there is no significant difference.However,the total mating duration of NDS was significant lower than that of DS.The mating frequency of DS was 3.8 ± 0.5 times which was significant higher than that of NDS (2.2 ± 0.3 times).Comparison of the mating behavior between DS and NDS shows that the reduced fecundity of NDS is associated with the mating behavior variation,including decreased mating rate,shortened mating duration and reduced mating frequency.The application of NDS can promote the study of the mechanism of insect diapause inheritance and regulation.%大猿叶虫Colaphellus bowringi Blay是一种重要的十字花科蔬菜害虫,以成虫越冬和越夏.明确大猿叶虫非滞育品系生殖力下降的现象是否与交配行为的改变相关,将促进该非滞育品系在滞育遗传、滞育调控分子机制研究中的应用.本研究在25℃和L:D=12:12 h条件下,测定了大猿叶虫滞育品系和非滞育品系的交配节律、交配持续时间和交配频次.结果表明,大猿叶虫滞育品系和非滞育品系交配高峰均出现在光期开始后2h,非滞育品系的交配比率显著低于滞育品系.滞育品系和非滞育品系第1次交配持续时间无显著差异,分别为122.7±7.4 min和100.1±10.2 min;但非滞育品系的总交配持续时间显著低于滞育品系.非滞育品系的交配频次为2.2±0.3次,显著低于滞育品系的3.8±0.5次.滞育品系和非滞育品系交配行为的比较发现,大猿叶虫非滞育品系生殖力降低与其交配比率下降、交配持续时间缩短和交配频次减少显著相关,本研究为进一步利用非滞育品系为材料解析滞育遗传、滞育调控机制奠定了基础.