三物白散含药血清对胃癌MGC-803细胞增殖、凋亡及survivin基因表达的影响

摘要

Objective: To tudy the efection of Sanwubai Powder medicated serum on proliferation,apoptosis and survivin expression of gastric cancer cell lines MGC-803. Methods: MGC-803 in vitro was selected.lt was used the 5-Fu as the positive control. The proliferation and the apoptosis of the Sanwubai Powder containing serum on the MGC-803 with the low,the medium and the high dose were detected by the colorimetric MTT and acridine orange,EB staining methods. And mRNA expression of survivin were detected by RT-PCR method. Results: After Sanwubai Powder medicated serum treatment to Gastric cancer cell MGC-803,as Sanwubai Powder drug-containing serum drug concentration increasing and time extending, cell proliferation rates decreased, cell inhibition rate and the rate of apoptosis increased gradually, the expression of survivin protein was lower correspondly. Conclusion: Sanwubai Powder drug-containing serum can inhibite proliferation of human gastric cancer MGC-803 cell, promote cell apoptosis. The expression of survivin gene will reduce in the apoptotic process.%目的 研究三物白散含药血清对人胃癌MGC-803细胞株的增殖、凋亡及survivin基因表达的影响.方法 体外实验选择MGC-803细胞,以5-氟尿嘧啶为阳性对照,采用MTT比色及吖啶橙、EB染色法检测低、中、高3组三物白散大鼠含药血清对MGC-803细胞增殖、凋亡的影响,运用RT-PCR法检测survivin mRNA的表达.结果 三物白散含药血清作用MGC-803细胞后,随着三物白散含药血清药物浓度的增加及作用时间的延长,细胞增殖率逐渐降低,细胞抑制率、凋亡率逐渐升高,survivin蛋白的表达相应的降低.结论 三物白散含药血清能够抑制人胃癌MGC-803细胞增殖,对细胞有诱导凋亡作用,在凋亡过程中涉及survivin基因表达下降.