IL-17A、TNF-α在哮喘小鼠模型气道炎症中的变化及射干麻黄汤的干预作用研究

摘要

Objective:To investigate the changes of IL-17A,TNF-α in airway inflammation exacerbation of chronic asthmatic mice model after Shegan Mahuang decoction treatment.Methods:Sixty mice were randomized into four groups:asthma group (group A),Shegan Mahuang decoction group (group B),blank group (group C),and dexamethasone group (group D).Mice were sensitized and challenged with ovalbumin (OVA),aluminum hydroxide combined with aerosol inhalation to establish the asthmatic models.The mice models of asthma underwent different therapies after models of asthma were established.The changes in cellular proportions in the bronchoalveolar lavage fluid (BALF) were observed,and the expression of IL-17 and TNF-α in BALF were detected by ELISA.Results:Compared with group C,messy and bleak hair,less movement and eating and slow response were observed during the experiment and during the aerosol inhalation,agitation,nose scratch,polypnea,nodding respiration and cyanotic lips at different degrees were observed in the mice in A,B,D groups.A group was more serious than B and D groups on the symptoms above.There was one death in group A and group D and no death in group B and group C.Compared with group C,The total cell number and the percentages of neutrophils (NEU) eosinophils (EOS) and lymphocytes (LYM) in BALF of the asthmatic mice were significantly higher in group A (P＜ 0.01),The changes in group B,D were less than group A (all P＜ 0.05),There was no significant difference between B and D(P＞0.05).The level of IL-17A,TNF-α in BALF of group A was significantly higher than those in group C(P＜0.05),The changes in group B,D were less than group A(all P＜0.05).There was no significant difference between B and D (P＞0.05).It showed that IL-17A and TNF-α played an important part in asthma airway inflammation mediation.Conclusion:Shegan Mahuang decoction can regress airway inflammation exacerbation of chronic asthmatic mice model,its mechanism may be achieved through reducing the expression of IL-17Aand TNF-α.%目的 观察细胞因子白介素-17A(IL-17A)、肿瘤坏死因子-α(TNF-α)在慢性哮喘小鼠模型气道炎症中的变化及射干麻黄汤的干预作用.方法 60只Balb/c小鼠小鼠随机分为模型组(A组)、中药汤剂组(B组)、对照组(C组)、地塞米松组(D组)4组.采用卵蛋白(OVA)、氢氧化铝联合雾化吸入的方法建立慢性哮喘小鼠模型,并通过不同方式对各组进行干预.取离心后的支气管肺泡灌洗液(BALF)中沉渣计细胞总数及细胞分类计数,取上清液以酶联免疫吸附试验(ELISA)法检测细胞因子IL-17A、TNF-α的含量.观察IL-17A、TNF-α在慢性哮喘小鼠模型气道炎症中的变化及射干麻黄汤的干预作用.结果 与C组相比,A、B、D组小鼠在实验期间均出现被毛凌乱、少光泽、活动及进食减少、反应迟钝等表现,在雾化激发时均出现不同程度的躁动、骚鼻、呼吸急促、点头样呼吸、口唇青紫等症状,其中B、D组上述症状明显较A组轻.实验过程中A、D组小鼠各死亡1例,B、C组无死亡.与C组比较,A组BALF中细胞总数及NEU、EOS、LYM百分率均显著增多(均P＜0.01);与A组比较,B、D组细胞数及NEU、EOS、LYM百分率均降低(均P＜0.05).D组与B组比较差别不大P＞ 0.05).与C组比较,A组细胞因子IL-17A、TNF-α含量增高(P＜0.05).与A组比较,B、D组细胞因子IL-17A、TNF-α含量均减少(均P＜ 0.05);D组与B组比较差别不大(P＞0.05).提示细胞因子IL-17、TNF-α在介导哮喘气道炎症反应中发挥重要作用.IL-17A、TNF-α与气道炎症均呈显著正相关(P＜0.05).结论 射干麻黄汤可能通过下调细胞因子IL-17A、TNF-α来抑制炎症细胞在哮喘小鼠气道内的聚集,从而减轻气道炎症.