红花注射液通过抗炎症反应治疗急性脑梗死的机制研究

摘要

目的 观察红花注射液对于脑卒中大鼠模型血清中超敏C反应蛋白(hs-CRP)炎性生物标志物表达的影响.方法 用改良线栓法制作大鼠中动脉阻断(MCAO)模型,评定红花注射液对MCAO大鼠神经功能缺损、脑梗死面积等的影响,并采用ELISA法来检测hs-CRP在脑卒中大鼠模型中表达的水平.结果 红花注射液能改善MCAO大鼠的的神经功能损伤情况;红花注射液12h组的脑梗死面积与模型12h组相比具有统计学意义(P＜0.05),红花注射液24 h组的脑梗死面积与模型24 h组相比具有统计学意义(P＜0.05).红花注射液12h组的hs-CRP与模型12h组相比具有均有统计学差异(P＜0.01).结论 红花注射液能减小急性脑梗死MCAO大鼠的脑梗死面积,减轻炎症反应,对于脑卒中大鼠模型血清中超敏C反应蛋白这种炎性生物标志物表达有抑制作用.%Objective:To explore the effect of safflower injection on the expression of inflammatory bio-markers of hypersensitive C-reactive protein in MCAO model rats.Methods:MCAO model was established with reforming Longa method.The effect of safflower injection on the neurological deficits and infarct size in MCAO rats was evaluated.The expression level of inflammatory bio-markers of hypersensitive C-reactive protein was detected using ELISA method.Results:Neurologic impairment in rats:safflower injection could improve the neurologic function of MCAO rats.The brain infarction area ratio:there was significant difference between the 12 hours group of safflower injection and the model group (P＜ 0.05),and there was also significant difference between the 24 hours group of safflower injection and the model group(P＜ 0.05).Serum hs-CRP concentration:there was significant difference between the hsCRP concentration of the safflower injection 12 hours group and the 12 hours model group (P＜ 0.05).Conclusion:Safflower injection can reduce the infarct area of the stroke model rats,relieve inflammatory reaction and inhibit the expression of inflammatory biomarkers such as serum super-sensitive C-reactive protein.