土家族药三百棒提取物对大鼠肢体缺血再灌注损伤血清SOD与MDA水平的影响

摘要

Objective:To investigate the protection of Toddalia asiatic extract in Tujia medicine in rats with limb ischemia reperfusion injury by observing the changes of serum SOD and MDA.Methods:66 SD rats were randomly divided into the control group,ischemia-reperfusion group and Toddalia asiatic extract intervention group.The control group were not given drug,and ischemia-reperfusion group were gavaged with 1 mL 0.9％ NS,in the first 12 h prior to ischemia and ischemia and reperfusion;the Toddalia asiatic extract intervention group in the first 12 h,ischemia and reperfusion were given 1 mL of Toddalia asiatic extracts.Inferior vena venous blood was extracted respectively in the corresponding time points in anesthesia to measure the contents of serum SOD and MDA.Results:Compared with ischemia-reperfusion group,serum SOD level in Toddalia asiatic extract in tervention group increased at each time point,while the MDA level significantly decreased (P＜ 0.05).Conclusions:Tujia medicine Toddalia asiatic extract can increase signal path expression of SOD,reduce pathways start of MDA with a protective effect on limb ischemia reperfusion injury in rats.%目的 观察血清超氧化物歧化酶(SOD)与丙二醛(MDA)变化,探寻三百棒提取物对大鼠肢体缺血再灌注损伤的保护作用机制.方法 66只SD大鼠随机分为对照组、缺血再灌注组和三百捧提取物干预组.对照组动物不给药物,缺血-再灌注组于缺血前12h、缺血时和再灌注时各给予0.9％氯化钠注射液1 mL灌胃;三百棒提取物干预组于缺血前12h、缺血时和再灌注时各给予三百棒提取物1 mL灌胃,分别于相应时间点在麻醉下抽取各组动物下腔静脉血,测量血清中SOD和MDA的含量.结果 与缺血-再灌注组相比,三百棒提取物干预组各时间点大鼠血清SOD水平明显上升,而MDA水平明显下降(P＜0.05).结论 三百棒提取物能增加SOD信号通路表达,减少MDA通路启动,对大鼠肢体缺血再灌注损伤具有保护作用.