为研究适用于水貂的专用菌制剂,试验采集健康成年母貂的新鲜肠粘膜和粪便,用改良MRS培养基,对肠道与粪便中的微生物进行分离培养,筛选优势菌株,观察其菌落形态,并进行生化试验和16S rRNA分子生物学鉴定,并对其生长情况和安全性等生物特性进行了研究.结果表明,优势菌株(RSJ)为乳酸杆菌；生长6h进入对数期,前18 h菌株繁殖较快,pH急速下降；18～36 h,菌株维持在稳定期；pH 2.0和3.0时菌株不生长,pH 5.0和6.0时生长良好；对青霉素和环丙沙星的耐药性较强,对其余的抗生素则表现为敏感；菌株RSJ对金黄色葡萄球菌、大肠杆菌均有抑菌作用.安全性试验显示,RSJ对小鼠无毒性.RSJ菌株具备了益生菌的基本条件,为进一步研究与开发微生态制剂奠定了基础.%In order to investigate the mink special beneficial bacteria,fresh intestinal mucosa and feces were taken by sterile from a healthy adult female mink in this test.Its intestinal and fecal microbes were isolated and cultured through improved MRS medium,a predominant strain was screened and named RSJ,Its colony morphology,microscopic characteristics,biochemical test were detected and was identified by 16S rRNA molecular biological experiments.Also the biological characteristics of the growth and security were studied.The results show that:the RSJ strain is Lactobacillus.The growth situation results showed that:the strain growed into logarithmic phase by liquid culture 6h,by 18h strain breeding faster.The value of pH was the rapid decline; 18-36 h,strains grew into a stable period.Acid-resistant test results showed that:the strain didn't grow at pH values of 2.0 and 3.0,strains grew well at the pH values of 5.0 and 6.0.Susceptibility testing and antibacterial test results showed that:the RSJ strain had strong resistance to penicillin and ciprofloxacin,not sensitive to the rest of the antibiotics; RSJ strain had significant inhibitory effect to Staphylococcus aureus and Escherichia coli.The safety trials showed that:RSJ strain was not toxic for mice.RSJ probiotic strains have the basic conditions and foundation for further research and development of probiotics.
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